Informace o publikaci

An improved method for the visualization of conductive vessels in Arabidopsis thaliana inflorescence stems

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JUPA Radek DIDI Vojtěch HEJÁTKO Jan GLOSER Vít

Rok publikování 2015
Druh Článek v odborném periodiku
Časopis / Zdroj Frontiers in Plant Science
Fakulta / Pracoviště MU

Přírodovědecká fakulta

Citace
www http://dx.doi.org/10.3389/fpls.2015.00211
Doi http://dx.doi.org/10.3389/fpls.2015.00211
Obor Fyziologie
Klíčová slova conductive elements; dye perfusion; fluorescence; Fluorescent Brightener 28; hydraulic conductivity; vessel; xylem
Popis Dye perfusion is commonly used for the identification of conductive elements important for the study of xylem development as well as precise hydraulic estimations. The tiny size of inflorescence stems, the small amount of vessels in close arrangement, and high hydraulic resistivity delimit the use of the method for quantification of the water conductivity of Arabidopsis thaliana, one of the recently most extensively used plant models. Here, we present an extensive adjustment to the method in order to reliably identify individual functional (conductive) vessels.

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