Informace o publikaci
SENSITIVE DETERMINATION OF GLUTATHIONE IN VARIOUS BIOLOGICAL SAMPLES BY CAPILLARY ELECTROPHORESIS WITH GREEN LASER INDUCED FLUORESCENCE DETECTION
| Autoři | |
|---|---|
| Rok publikování | 2014 |
| Druh | Článek ve sborníku |
| Konference | CECE 2014: 11TH INTERNATIONAL INTERDISCIPLINARY MEETING ON BIOANALYSIS |
| Fakulta / Pracoviště MU | |
| Citace | |
| Obor | Analytická chemie, separace |
| Klíčová slova | GLUTATHIONE; fluorescence |
| Popis | Laser induced fluorescence capillary electrophoresis (CE-LIF) with 515 nm green laser module was used for sensitive determination of glutathione (GSH) in various biological samples. For the first time, eosin-5-maleimide was used as a fluorescent tag in CE. Studies were performed to optimize the derivatization conditions (the ratio of reagent to analyte, the reaction time, temperature, etc.). CE-LIF also offers a unique opportunity to monitor the stability of the reagent and various reaction products. We demonstrate the applicability of the developed method on determination of GSH in real biological samples (exhaled breath condensate and saliva) with LODs as low as 180 pM. |