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Toward Structured Macroporous Hydrogel Composites: Electron Beam-Initiated Polymerization of Layered Cryogels

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GOLUNOVA Anna CHVÁTIL David KRIST Pavel JAROŠ Josef JURTÍKOVÁ Veronika POSPÍŠIL Jakub KOTELNIKOV Ilya ABELOVÁ Lucie KOTEK Jiří SEDLAČÍK Tomáš KUČKA Jan KOUBKOVÁ Jana STUDENOVSKÁ Hana STREIT Libor HAMPL Aleš RYPÁČEK František PROKS Vladimír

Rok publikování 2015
Druh Článek v odborném periodiku
Časopis / Zdroj Biomacromolecules
Fakulta / Pracoviště MU

Lékařská fakulta

Citace
Doi http://dx.doi.org/10.1021/bm501809t
Obor Biochemie
Klíčová slova STEM-CELLS; POLYSACCHARIDE HYDROGELS; MECHANICAL-PROPERTIES; EXTRACELLULAR-MATRIX; TISSUE REGENERATION; AQUEOUS-SOLUTIONS; ADIPOSE-TISSUE; DRUG-DELIVERY; DIFFERENTIATION; SCAFFOLDS
Popis The ability to tailor mechanical properties and architecture is crucial in creating macroporous hydrogel scaffolds for tissue engineering. In the present work, a technique for the modification of the pore size and stiffness of acrylamide-based cryogels is demonstrated via the regulation of an electron beam irradiation dose. The samples were characterized by equilibrium swelling measurements, light and scanning electron microscopy, mercury porosimetry, Brunauer-Emmett-Teller surface area analysis, and stiffness measurements. Their properties were compared to cryogels prepared by a standard redox-initiated radical polymerization. A I-125 radiolabeled azidopentanoyl-GGGRGDSGGGY-NH2 peptide was bound to the surface to determine the concentration of the adhesive sites available for biomimetic modification. The functionality of the prepared substrates was evaluated by in vitro cultivation of adipose-derived stem cells. Moreover, the feasibility of preparing layered cryogels was demonstrated. This may be the key to the future preparation of complex hydrogel-based scaffolds to mimic the extracellular microenvironment in a wide range of applications.
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