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Prevalence study on carbapenemase-producing Escherichia coli and Klebsiella pneumoniae isolates in Czech hospitals - results from Czech Part of European Survey on Carbapenemase-Producing Enterobacteriaceae (EuSCAPE)

Název česky prevalenční studie kmenů Escherichia coli a Klebsiella pneumoniae produkujících karbapenemázu v českých nemocnicích – výsledky české části studie euSCApe (european Survey on Carbapenemase-producing enterobacteriaceae)
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HRABÁK J. ŠTUDENTOVÁ V. JAKUBŮ V. ADÁMKOVÁ V. DVOŘÁKOVÁ L. BALEJOVÁ M. BERGEROVÁ T. CHMELAŘOVÁ E. JEŽEK P. KABELÍKOVÁ P. KOLÁŘ M. PATEROVÁ P. TEJKALOVÁ Renata PAPAGIANNITSIS C. ŽEMLIČKOVÁ H.

Rok publikování 2015
Druh Článek v odborném periodiku
Časopis / Zdroj Epidemiologie, mikrobiologie, imunologie
Fakulta / Pracoviště MU

Lékařská fakulta

Citace
Obor Mikrobiologie, virologie
Klíčová slova carbapenemase; CPE; MALDI-TOF; susceptibility; resistance; Enterobacteriaceae
Popis Objective: One of the most important threats of current medicine is the spread of multiresistant Gram-negative bacteria. We report here data from a six-month prevalence study on carbapenemase-producing K. pneumoniae and E. coli performed in Czech hospitals participating on European Survey on Carbapenemase-Producing Enterobacteriaceae (EuSCAPE). Methods: Ten hospitals covering all regions of the Czech Republic were selected. During the study period (1st November 2013 to 30th April 2014), first ten carbapenem non-susceptible isolates of K. pneumoniae or E. coli isolated from non-surveillance specimens (i.e., blood, lower respiratory tract secretions, urine, puncture fluids, and wound secretions) of single successive patients were collected. Successive carbapenem-susceptible isolates of the same species were also preserved as controls. Susceptibility to 15 antibiotics was determined using EUCAST recommendations. Carbapenemase activity was detected by MALDI-TOF MS meropenem hydrolysis assay. Positive isolates were subjected for molecular typing (multi-locus sequence typing, identification of carbapenemase gene). Results: During the study period, thirty non-susceptible isolates (K. pneumoniae n = 28, E. coli n = 2) were identified in 5 hospitals. Only two of them were confirmed to be carbapenemase producers. A NDM-1-producing K. pneumoniae ST11 was recovered from a patient, transferred from Ukraine, being injured during a Maidan revolution. The second isolate, an OXA-48-producing K. pneumoniae, belonging to ST101, was recovered from a patient admitted to a hospital for an ischemic stroke. Conclusions: This study again confirmed that the Czech Republic still belongs to the countries with low prevalence of carbapenemase-producing Enterobacteriaceae (CPE). Cases of CPE are usually restricted to an import from high-prevalence countries or countries with unknown epidemiological situation.

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