Informace o publikaci

Relation of exposure to amino acids involved in sarcosine metabolic pathway on behavior of non-tumor and malignant prostatic cell lines

Autoři	HEGER Zbynek GUMULEC Jaromír CERNEI Natalia POLANSKÁ Hana RAUDENSKÁ Martina MASAŘÍK Michal ECKSCHLAGER Tomas STIBOROVA Marie ADAM Vojtech KIZEK Rene
Rok publikování	2016
Druh	Článek v odborném periodiku
Časopis / Zdroj	Prostate
Fakulta / Pracoviště MU	Lékařská fakulta
Citace
Doi	http://dx.doi.org/10.1002/pros.23159
Obor	Onkologie a hematologie
Klíčová slova	cancer metabolism; dimethylglycine; folate; glycine; sarcosine pathway; prostate cancer
Popis	BACKGROUND Sarcosine (N-methylglycine) was previously delineated as a substantial oncometabolite of prostate cancer (PCa) and its metabolism seems to be significantly involved in PCa development and behavior. METHODS We focused on investigation whether the exposure of prostate cells (PNT1A, 22Rv1, and PC-3) to sarcosine-related amino acids (glycine, dimethylglycine, and sarcosine) affects their aggressiveness (cell mobility and division rates, using real-time cell based assay). The effect of supplementation on expression of glycine-N-methyltransferase (GNMT) mRNA was examined using qRT-PCR. Finally, post-treatment amino acids patterns were determined with consequent statistical processing using the Ward's method, factorial ANOVA and principal component analysis (P<0.05). RESULTS The highest migration induced sarcosine and glycine in metastatic PC-3 cells (a decrease in relative free area about 53% and 73%). The highest cell division was achieved after treatment of 22Rv1 and PC-3 cells with sarcosine (time required for division decreased by 65% or 45%, when compared to untreated cells). qRT-PCR revealed also significant effects on expression of GNMT. Finally, amino acid profiling shown specific amino acid patterns for each cell line. In both, treated and untreated PC-3 cells significantly higher levels of serine, glutamic acid, and aspartate, linked with prostate cancer progression were found. CONCLUSIONS Sarcosine-related amino acids can exceptionally affect the behavior of benign and malignant prostate cells.