Eudiplozoon nipponicum (Heteronchoinea: Diplozoidae): insight into composition of E/S products and basic characterization of serpin

• Autoři: ROUDNICKÝ Pavel; VOREL Jiří; ILGOVÁ Jana; MIKEŠ Libor; JEDLIČKOVÁ Lucie; DALTON John; DVOŘÁK Jan; ZDRÁHAL Zbyněk; POTĚŠIL David; JANDA Lubomír; NOREK Adam; GELNAR Milan; KAŠNÝ Martin
• Rok publikování: 2016
• Druh: Další prezentace na konferencích
• Fakulta / Pracoviště MU: Přírodovědecká fakulta
• Popis: Two genes coding serpins, were expressed in E. coli system (BL21 strain) in combination with synthetic genes, optimised for this system, using pET-22B(+) expression vector. IPTG induced expression (30°C, overnight) was analyzed on SDS-PAGE and soluble recombinant serpins of E. nipponicum was partially purified using Ni-NTA agarose beads. Successful production of serpins was verified by mass spectrometry and Western blot using His-tag primary antibodies.

Související projekty

• ECIP - Evropské centrum ichtyoparazitologie