Informace o publikaci

Asymmetric dimethyl arginine induces pulmonary vascular dysfunction via activation of signal transducer and activator of transcription 3 and stabilization of hypoxia-inducible factor 1-alpha

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PEKAROVÁ Michaela KOUDELKA Adolf KOLÁŘOVÁ Hana AMBROŽOVÁ Gabriela KLINKE Anna ČERNÁ Anna KADLEC Jaroslav TRUNDOVÁ Mária SVIHALKOVA Lenka Sindlerova KUCHTA Radek KUCHTOVA Zdenka LOJEK Antonín KUBALA Lukáš

Rok publikování 2015
Druh Článek v odborném periodiku
Časopis / Zdroj Vascular Pharmacology
Citace
www http://www.sciencedirect.com/science/article/pii/S1537189115001366
Doi http://dx.doi.org/10.1016/j.vph.2015.06.005
Klíčová slova Asymmetric dimethyl arginine; Pulmonary hypertension; Human pulmonary artery endothelial cell; Human pulmonary artery smooth muscle cell; Hypoxia
Popis Pulmonary hypertension (PH), associated with imbalance in vasoactive mediators and massive remodeling of pulmonary vasculature, represents a serious health complication. Despite the progress in treatment, PH patients typically have poor prognoses with severely affected quality of life. Asymmetric dimethyl arginine (ADMA), endogenous inhibitor of endothelial nitric oxide synthase (eNOS), also represents one of the critical regulators of pulmonary vascular functions. The present study describes a novel mechanism of ADMA-induced dysfunction in human pulmonary endothelial and smooth muscle cells. The effect of ADMA was compared with well-established model of hypoxia-induced pulmonary vascular dysfunction. It was discovered for the first time that ADMA induced the activation of signal transducer and activator of transcription 3 (STAT3) and stabilization of hypoxia inducible factor 1? (HIF-1?) in both types of cells, associated with drastic alternations in normal cellular functions (e.g., nitric oxide production, cell proliferation/Ca2+ concentration, production of pro-inflammatory mediators, and expression of eNOS, DDAH1, and ICAM-1). Additionally, ADMA significantly enhanced the hypoxia-mediated increase in the signaling cascades. In summary, increased ADMA may lead to manifestation of PH phenotype in human endothelial and smooth muscle cells via the STAT3/HIF-1? cascade. Therefore this signaling pathway represents the potential pathway for future clinical interventions in PH.

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