Informace o publikaci

"Positive Regulation of RNA Metabolic Process" Ontology Group Highly Regulated in Porcine Oocytes Matured In Vitro: A Microarray Approach

Autoři	CELICHOWSKI Piotr NAWROCKI Mariusz J. DYSZKIEWICZ-KONWINSKA Marta JANKOWSKI Maurycy BUDNA Joanna BRYJA Artur KRANC Wieslawa BORYS Sylwia KNAP Sandra CIESIOLKA Sylwia JEŠETA Michal PIASECKA-STRYCZYNSKA Karolina KHOZMI Ronza BUKOWSKA Dorota ANTOSIK Pawel BRUSSOW Klaus P. BRUSKA Malgorzata NOWICKI Michal ZABEL Maciej KEMPISTY Bartosz
Rok publikování	2018
Druh	Článek v odborném periodiku
Časopis / Zdroj	Biomed Research International
Fakulta / Pracoviště MU	Lékařská fakulta
Citace
Doi	http://dx.doi.org/10.1155/2018/2863068
Klíčová slova	RNA Metabolic Process
Popis	The cumulus-oocyte complexes (COCs) growth and development during folliculogenesis and oogenesis are accompanied by changes involving synthesis and accumulation of large amount of RNA and proteins. In this study, the transcriptomic profile of genes involved in "oocytes RNA synthesis" in relation to in vitro maturation in pigs was investigated for the first time. The RNA was isolated from oocytes before and after in vitro maturation (IVM). Interactions between differentially expressed genes/ proteins belonging to "positive regulation of RNA metabolic process" ontology group were investigated by STRING10 software. Using microarray assays, we found expression of 12258 porcine transcripts. Genes with fold change higher than \|2\| and with corrected.. value lower than 0.05 were considered as differentially expressed. The ontology group "positive regulation of RNA metabolic process" involved differential expression of AR, INHBA, WWTR1, FOS, MEF2C, VEGFA, IKZF2, IHH, RORA, MAP3K1, NFAT5, SMARCA1, EGR1, EGR2, MITF, SMAD4, APP, and NR5A1 transcripts. Since all of the presented genes were downregulated after IVM, we suggested that they might be significantly involved in regulation of RNA synthesis before reaching oocyte MII stage. Higher expression of "RNA metabolic process" related genes before IVM indicated that they might be recognized as important markers and specific "transcriptomic fingerprint" of RNA template accumulation and storage for further porcine embryos growth and development.