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Resistive Pulse Sensing as Particle Counting and Sizing Method in Microfluidic Systems: Designs and Applications Review

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VÁCLAVEK Tomáš PŘIKRYL Jan FORET František

Rok publikování 2019
Druh Článek v odborném periodiku
Časopis / Zdroj Journal of separation science
Fakulta / Pracoviště MU

Přírodovědecká fakulta

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Doi http://dx.doi.org/10.1002/jssc.201800978
Klíčová slova bioanalysis;electrical impedance spectroscopy;microfluidics;single cell analysis
Popis Resistive pulse sensing is a well-known and established method for counting and sizing particles in ionic solutions. Throughout its development the technique has been expanded from detection of biological cells to counting nanoparticles and viruses, and even registering individual molecules, e.g., nucleotides in nucleic acids. This technique combined with microfluidic or nanofluidic systems shows great potential for various bioanalytical applications, which were hardly possible before microfabrication gained the present broad adoption. In this review, we provide a comprehensive overview of microfluidic designs along with electrode arrangements with emphasis on applications focusing on bioanalysis and analysis of single cells that were reported within the past five years.
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