Informace o publikaci
Ampérometrický biosenzor pre rýchle stanovenie albumínu v moči
| Autoři | |
|---|---|
| Rok publikování | 2019 |
| Druh | Konferenční abstrakty |
| Fakulta / Pracoviště MU | |
| Citace | ŠIŠKOVÁ, Martina, Matěj PASTUCHA, Marta FARKOVÁ, Přemysl LUBAL a Zdeněk FARKA. Ampérometrický biosenzor pre rýchle stanovenie albumínu v moči. In 22. ročník celostátní soutěže o nejlepší studentskou vědeckou práci v oboru analytická chemie Cena Karla Štulíka 2019. 2019. ISSN 2336-7202. |
| Popis | This work presents the development of an amperometric biosensor for the rapid determination of clinically relevant concentrations of human serum albumin in the urine, whose increased concentration indicates nephropathy. This simple method is characterized by a short analysis time, low cost of determination, possibility of miniaturization, automatization and point of care analysis. The assay was performed by using various methods of immobilization the antibody to screen-printed electrodes by means of mixed and flow-through configuration. By immobilizing the antibody via cysteamine and glutaraldehyde in a mixed analysis, a detection limit of 10 ng ml–1 was reached, allowing the determination of human serum albumin in the urine. |
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