Informace o publikaci

Genes Involved in the Processes of Cell Proliferation, Migration, Adhesion, and Tissue Development as New Potential Markers of Porcine Granulosa Cellular Processes In Vitro: A Microarray Approach

Autoři	OZEGOWSKA Katarzyna BRAZERT Maciej CIESIOLKA Sylwia NAWROCKI Mariusz J. KRANC Wieslawa CELICHOWSKI Piotr JANKOWSKI Maurycy BRYJA Artur JEŠETA Michal ANTOSIK Pawel BUKOWSKA Dorota SKOWRONSKI Mariusz T. BRUSKA Malgorzata PAWELCZYK Leszek ZABEL Maciej NOWICKI Michal KEMPISTY Bartosz
Rok publikování	2019
Druh	Článek v odborném periodiku
Časopis / Zdroj	DNA and Cell Biology
Fakulta / Pracoviště MU	Lékařská fakulta
Citace
www	http://dx.doi.org/10.1089/dna.2018.4467
Doi	http://dx.doi.org/10.1089/dna.2018.4467
Klíčová slova	porcine granulosa cells; In Vitro Maturation (IVM); cellular migration; proliferation; adhesion
Popis	Proper course of folliculogenesis and oogenesis have an enormous impact on female fertility. Both processes take place in the ovary and involve not only the maturing germ cell, but also few types of somatic cells that assist the ovarian processes and mediate the dialog with the oocyte. These cells, granulosa and theca, are heavily involved in essential reproductive processes, such as ovulation, fertilization, and embryo implantation. In this study, we have used the expressive microarray approach to analyze the transcriptome of porcine granulosa cells, during short-term in vitro culture. We have further selected differentially expressed gene ontologies, involved in cell proliferation, migration, adhesion, and tissue development, namely, "cell-cell adhesion," "cell motility," "cell proliferation," "tissue development," and "tissue migration" to screen them for the possibility of discovery of new markers of those processes. A total of 303 genes, expression of which varied significantly in different culture periods and belonged to the analyzed ontology groups, were detected, of which 15 that varied the most (between 0 and 48 h of culture) were selected for validation. As the validation confirmed the transcriptomic patterns, 10 genes of biggest changes in expression (CAV1, IGFBP5, ITGB3, FN1, ITGA2, LAMB1, POSTN, FAM83D, KIF14, and CDK1) were analyzed, described, and referred to the context of the study, with the most promising new markers and further proof for the viability of the currently recognized ones detailed. Overall, the study provided valuable insight into the molecular functioning of in vitro granulosa cell cultures.