Informace o publikaci

Cross-linker effects on the separation efficiency on (poly)methacrylate capillary monolithic columns. Part II. Aqueous normal-phase liquid chromatography

Autoři	STANKOVA M JANDERA P SKERIKOVA V URBAN Jiří
Rok publikování	2013
Druh	Článek v odborném periodiku
Časopis / Zdroj	Journal of Chromatography A
Citace
Doi	http://dx.doi.org/10.1016/j.chroma.2013.03.025
Klíčová slova	Organic-polymer momoliths; HILIC; Column efficiency; Capillary HPLC; Pore size distribution
Popis	We synthesized seven different polymethacrylate monolithic capillary columns using N,N-dimethyl-N-metacryloxyethyl-N-(3-sulfopropyl) ammonium betaine functional monomer (MEDSA) and various cross-linking monomers differing in the polarity and size. The efficiency of monolithic columns for polar low-molecular compounds in the aqueous normal-phase (HILIC) mode depends rather on the polarity, than on the size of the cross-linker molecules. Cross-linking molecules, which exhibit high polarity can produce poly(methacrylate) monoliths with an increase in pore sizes below 50 nm. Columns prepared with pentaerythritol triacrylate or bisphenol A dimethacrylate cross-linkers show large inner pore (mesopore) porosity, and provide poor efficiency, whereas columns with trioxyethylene dimethacrylate (TriEDMA) and tetraoxyethylene dimethacrylate (TeEDMA) cross-linkers showed poor permeability. Columns prepared using dioxyethylene dimethacrylate (DiEDMA), and especially glycerolate dimethacrylate (BIGDMA) cross-linkers, showed best efficiency, with more 60,000-70,000 theoretical plates/m, almost twice in comparison to (poly)methylene dimethacrylate (HEDMA) in the HILIC mode. All columns show dual retention mechanism and can be used for separations of low-molcular compounds such as phenolic acids in the HILIC mode in acetonitrile-rich mobile phases and in the reversed-phase mode in mobile phases with higher concentrations of water. Some columns show broad pore distribution and can be used for size-exclusion chromatography of non-polar polymers in tetrahydrofuran. (c) 2013 Elsevier B.V. All rights reserved.