Informace o publikaci

Small non-coding RNA profiling in breast cancer: plasma U6 snRNA, miR-451a and miR-548b-5p as novel diagnostic and prognostic biomarkers

Autoři	ZAVESKY Ludek JANDÁKOVÁ Eva WEINBERGER Vít MINÁŘ Luboš HANZIKOVA Veronika DUSKOVA Daniela FARIDOVA Adela TURYNA Radovan SLANAR Ondrej HORINEK Ales KOHOUTOVA Milada
Rok publikování	2022
Druh	Článek v odborném periodiku
Časopis / Zdroj	MOLECULAR BIOLOGY REPORTS
Fakulta / Pracoviště MU	Lékařská fakulta
Citace
www	https://link.springer.com/article/10.1007%2Fs11033-021-07010-8
Doi	http://dx.doi.org/10.1007/s11033-021-07010-8
Klíčová slova	Biomarker; Breast cancer; microRNA; miR-451a; miR-548b-5p; U6 snRNA
Popis	Background Breast cancer is a leading cause of cancer-related death in women. Most cases are invasive ductal carcinomas of no special type (NST breast carcinomas). Methods and Results In this prospective, multicentric biomarker discovery study, we analyzed the expression of small non-coding RNAs (mainly microRNAs) in plasma by qPCR and evaluated their association with NST breast cancer. Large-scale expression profiling and subsequent validations have been performed in patient and control groups and compared with clinicopathological data. Small nuclear U6 snRNA, miR-548b-5p and miR-451a have been identified as candidate biomarkers. U6 snRNA was remarkably overexpressed in all the validations, miR-548b-5p levels were generally elevated and miR-451a expression was mostly downregulated in breast cancer groups. Combined U6 snRNA/miR-548b-5p signature demonstrated the best diagnostic performance based on the ROC curve analysis with AUC of 0.813, sensitivity 73.1% and specificity 82.6%. There was a trend towards increased expression of both miR-548b-5p and U6 snRNA in more advanced stages. Further, increased miR-548b-5p levels have been partially associated with higher grades, multifocality, Ki-67 positivity, and luminal B rather than luminal A samples. On the other hand, an association has been observed between high miR-451a expression and progesterone receptor positivity, lower grade, unifocal samples, Ki-67-negativity, luminal A rather than luminal B samples as well as improved progression-free survival and overall survival. Conclusions Our results indicated that U6 snRNA and miR-548b-5p may have pro-oncogenic functions, while miR-451a may act as tumor suppressor in breast cancer.