Informace o publikaci
Elegant, rapid and simple procedure for labeling of antibodies with fluorescent ZnCd/S quantum dots for laser ablation ICP-MS detection
| Autoři | |
|---|---|
| Rok publikování | 2021 |
| Druh | Článek ve sborníku |
| Konference | 12th International Conference on Nanomaterials - Research and Application, NANOCON 2020 |
| Fakulta / Pracoviště MU | |
| Citace | |
| www | https://www.confer.cz/nanocon/2020/3752-elegant-rapid-and-simple-procedure-for-labeling-of-antibodies-with-fluorescent-cdzn-s-nanoparticles-for-la-icp-ms-detection |
| Doi | http://dx.doi.org/10.37904/nanocon.2020.3752 |
| Klíčová slova | Antibody; antigen; quantum dots; cleavage disulfide bonds; fluorescent |
| Popis | Antibody labeling with a signal-providing tag (e.g. an enzyme, chromophore, fluorophore, metal chelate, radioisotope, etc.) is a key procedure in bioanalytical techniques such an enzyme-linked immunosorbent assay, western blot, or immunohistochemical analysis. The process may be laborious, impair the activity, and time-consuming. Concisely, in this work, an elegant method of rapid and effective antibody labeling by quantum dots was developed taking advantage of partial reduction of antibody structure and UV-induced quantum dot formation. Zinc and cadmium with the thiol group of the antibody as capping agents stabilizing the fluorescent nanoparticle while the biorecognition capabilities were maintained. The ZnCd/S quantum dots creation has to prosper detected with capillary electrophoresis (CE) and laser ablation ICP- MS (LA- ICP- MS). |