Informace o publikaci

Complex Alterations of Fatty Acid Metabolism and Phospholipidome Uncovered in Isolated Colon Cancer Epithelial Cells

Autoři	HOFMANOVÁ Jiřina SLAVIK Josef CIGANEK Miroslav OVESNÁ Petra TYLICHOVA Zuzana KARASOVÁ Martina ZAPLETAL Ondrej STRAKOVA Nicol PROCHAZKOVA Jirina BOUCHAL Jan KOLAR Zdenek EHRMANN Jiri LEVKOVA Monika HUSKOVA Zlatka SKALICKY Pavel KOZUBÍK Alois MACHALA Miroslav VONDRÁČEK Jan
Rok publikování	2021
Druh	Článek v odborném periodiku
Časopis / Zdroj	International Journal of Molecular Sciences
Fakulta / Pracoviště MU	Přírodovědecká fakulta
Citace
www	https://www.mdpi.com/1422-0067/22/13/6650
Doi	http://dx.doi.org/10.3390/ijms22136650
Klíčová slova	phospholipids; lysophospholipids; fatty acid synthesis; desaturation; colorectal carcinoma; EpCAM; epithelial cells; lipidomics
Popis	The development of colon cancer, one of the most common malignancies, is accompanied with numerous lipid alterations. However, analyses of whole tumor samples may not always provide an accurate description of specific changes occurring directly in tumor epithelial cells. Here, we analyzed in detail the phospholipid (PL), lysophospholipid (lysoPL), and fatty acid (FA) profiles of purified EpCAM(+) cells, isolated from tumor and adjacent non-tumor tissues of colon cancer patients. We found that a number of FAs increased significantly in isolated tumor cells, which also included a number of long polyunsaturated FAs. Higher levels of FAs were associated with increased expression of FA synthesis genes, as well as with altered expression of enzymes involved in FA elongation and desaturation, including particularly fatty acid synthase, stearoyl-CoA desaturase, fatty acid desaturase 2 and ELOVL5 fatty acid elongase 5 We identified significant changes in ratios of specific lysoPLs and corresponding PLs. A number of lysophosphatidylcholine and lysophosphatidylethanolamine species, containing long-chain and very-long chain FAs, often with high numbers of double bonds, were significantly upregulated in tumor cells. Increased de novo synthesis of very long-chain FAs, or, altered uptake or incorporation of these FAs into specific lysoPLs in tumor cells, may thus contribute to reprogramming of cellular phospholipidome and membrane alterations observed in colon cancer.