Informace o publikaci

Complete Genome Sequence of the Type Strain Tepidimonas taiwanensis LMG 22826T, a Thermophilic Alkaline Protease and Polyhydroxyalkanoate Producer

Autoři	HERMANKOVA Kristyna KOURILOVA Xenie PERNICOVA Iva BEZDÍČEK Matěj LENGEROVÁ Martina OBRUCA Stanislav SEDLAR Karel
Rok publikování	2021
Druh	Článek v odborném periodiku
Časopis / Zdroj	GENOME BIOLOGY AND EVOLUTION
Fakulta / Pracoviště MU	Lékařská fakulta
Citace
www	https://academic.oup.com/gbe/article/13/12/evab280/6462190
Doi	http://dx.doi.org/10.1093/gbe/evab280
Klíčová slova	Tepidimonas taiwanensis; polyhydroxyalkanoates; hybrid assembly; Oxford Nanopore Technologies; functional annotation; alkaline protease
Popis	Tepidimonas taiwanensis is a moderately thermophilic, Gram-negative, rod-shaped, chemoorganoheterotrophic, motile bacterium. The alkaline protease producing type strain T. taiwanensis LMG 22826T was recently reported to also be a promising producer of polyhydroxyalkanoates (PHAs)—renewable and biodegradable polymers representing an alternative to conventional plastics. Here, we present its first complete genome sequence which is also the first complete genome sequence of the whole species. The genome consists of a single 2,915,587-bp-long circular chromosome with GC content of 68.75%. Genome annotation identified 2,764 genes in total while 2,634 open reading frames belonged to protein-coding genes. Although functional annotation of the genome and division of genes into Clusters of Orthologous Groups (COGs) revealed a relatively high number of 694 genes with unknown function or unknown COG, the majority of genes were assigned a function. Most of the genes, 406 in total, were involved in energy production and conversion, and amino acid transport and metabolism. Moreover, particular key genes involved in the metabolism of PHA were identified. Knowledge of the genome in connection with the recently reported ability to produce bioplastics from the waste stream of wine production makes T. taiwanensis LMG 22826T, an ideal candidate for further genome engineering as a bacterium with high biotechnological potential.