Informace o publikaci

Early immune response of two common carp breeds to koi herpesvirus infection

Název česky	Raná imunitní odpověď dvou plemen kapra obecného na infekci koi herpesvirem
Autoři	MACHÁT Radek POJEZDAL Lubomir GEBAUER Jan MATIAŠOVIC Ján TESAŘÍK Radek MINAROVA Hana HODKOVICOVA Nikola FALDYNA Martin
Rok publikování	2022
Druh	Článek v odborném periodiku
Časopis / Zdroj	Fish and Shellfish Immunology
Fakulta / Pracoviště MU	Přírodovědecká fakulta
Citace
www	https://doi.org/10.1016/j.fsi.2022.07.064
Doi	http://dx.doi.org/10.1016/j.fsi.2022.07.064
Klíčová slova	qRT-PCR; Mass spectrometry; Koi carp; Amur wild carp; Complement cascade; Interferon class I; Cell-mediated cytotoxicity
Popis	Economic importance of common carp (Cyprinus carpio L.) increases every year. Viral diseases are major threat for carp aquaculture and cause significant economic losses. Koi herpesvirus (KHV) is one of the most serious carp diseases. Current study is focused on confirmation of possible differences in early immune response to KHV depending on level of resistance. Class I interferon signalling, complement cascade and cell-mediated cytotoxicity are hypothesized as major mechanisms of early innate immune response against KHV. Different breeds of common carp show distinct level of resistance to KHV. Two breeds of common carp with completely different susceptibility to KHV were chosen for current research: amur wild carp (AS) as highly resistant and koi carp (KOI) as very susceptible breed. KHV infection caused no mortalities, but the viral load in selected tissues increased during infection. Levels of expressions of chosen genes was examined using qRT-PCR and overall change in protein expression profiles was analysed by mass spectrometry. Significant differences in immune response between AS and KOI were detected mostly at the level of protein expression. Although cell-mediated cytotoxicity showed minimal influence during KHV infection, many immune response parameters related to class I interferon signalling pathway and complement cascade were increased earlier during KHV infection in AS comparing to KOI.