Informace o publikaci

Capillary electrophoretic methods for classification of methicillin-resistant Staphylococcus aureus (MRSA) clones

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HORKÁ Marie RŮŽIČKA Filip SIVÁKOVÁ Alena KARÁSEK Pavel ŠALPLACHTA Jiří PANTŮČEK Roman ROTH Michal

Rok publikování 2022
Druh Článek v odborném periodiku
Časopis / Zdroj Analytica Chimica Acta
Fakulta / Pracoviště MU

Přírodovědecká fakulta

Citace
www https://doi.org/10.1016/j.aca.2022.340305
Doi http://dx.doi.org/10.1016/j.aca.2022.340305
Klíčová slova Capillary electrophoresis; Classification; Fused silica capillary; Separation; Staphylococcus aureus; Temperature; MRSA
Přiložené soubory
Popis This study describes differentiation of methicillin-resistant Staphylococcus aureus (MRSA) isolates belonging to different genotype groups by the combination of electrophoretic techniques, transient isotachophoresis and micellar electrokinetic chromatography. MRSA isolates were separated in fused silica capillary with roughened inner surface prepared by etching with supercritical water. Separation temperature together with the rinsing procedure of the capillary turned out to be the key factors of successful analysis. The individual genotype groups were baseline-resolved in 40 min. Partial separation of the individual isolates within the groups was also observed. Relative standard deviations of the migration times of the isolate zones ranged from 0.32 to 0.79%. In addition, capability of the developed CE method to concentrate and separate MRSA isolates in clinical samples was proved by the analysis of blood sample.
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