Informace o publikaci
Direct detection of TNF-alpha by copper benzene tricarboxylate MOFs/gold nanoparticles modified electrochemical label-free immunosensor using FFT admittance voltammetry
| Autoři | |
|---|---|
| Rok publikování | 2022 |
| Druh | Článek v odborném periodiku |
| Časopis / Zdroj | Journal of Electroanalytical Chemistry |
| Fakulta / Pracoviště MU | |
| Citace | |
| www | https://www.sciencedirect.com/science/article/pii/S157266572200889X?via%3Dihub |
| Doi | http://dx.doi.org/10.1016/j.jelechem.2022.116897 |
| Klíčová slova | TNF-? Immunoassay; Metal organic framework; Label-free electrochemical immunosensor; Gold nanoparticles; Etanercept |
| Popis | A new simple label-free electrochemical immunosensor was designed for sensitive and selective determination of tumor necrosis factor-alpha (TNF-?), through gold nanoparticles (GNPs) incorporated copper benzene tricarboxylate metal organic frameworks (HKUST-1) modified gold electrode, where TNF-? specific monoclonal antibody was bonded to the electrode surface through shaped self-assembled monolayer (SAM) of mercapto compounds. The fabrication steps were monitored by field emission scanning electron microscopy (FE-SEM), cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS). Under optimized experimental conditions, fast Fourier transform admittance voltammetry (FFTADV) used for TNF-? measurement. A wide linear range of current response towards the TNF-? concentration from 0.0005 to 50 ng/mL and LOD value of 0.15 pg/mL were achieved using FFTADV. The described immunosensor had adequate sensitivity and selectivity towards direct determination of TNF-? in complex biological matrix. |