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Informace o publikaci
Mapping of MeLiM melanoma combining ICP-MS and MALDI-MSI methods
Autoři | |
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Rok publikování | 2022 |
Druh | Článek v odborném periodiku |
Časopis / Zdroj | International Journal of Biological Macromolecules |
Fakulta / Pracoviště MU | |
Citace | |
www | https://doi.org/10.1016/j.ijbiomac.2022.01.139 |
Doi | http://dx.doi.org/10.1016/j.ijbiomac.2022.01.139 |
Klíčová slova | Melanoma; Mass spectrometry; MeLiM |
Popis | Here we developed a powerful tool for comprehensive data collection and mapping of molecular and elemental signatures in the Melanoma-bearing Libechov Minipig (MeLiM) model. The combination of different mass spectrometric methods allowed for detail investigation of specific melanoma markers and elements and their spatial distribution in tissue sections. MALDI-MSI combined with HPLC-MS/MS analyses resulted in identification of seven specific proteins, S100A12, CD163, MMP-2, galectin-1, tenascin, resistin and PCNA that were presented in the melanoma signatures. Furthermore, the ICP-MS method allowed for spatial detection of zinc, calcium, copper, and iron elements linked with the allocation of the specific binding proteins. |