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Quality control of clinical-grade human embryonic stem cells

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SOURALOVÁ Tereza VENTRUBA Pavel JEŠETA Michal ŘEHÁKOVÁ Daniela HAMPL Aleš KOUTNÁ Irena

Rok publikování 2023
Druh Další prezentace na konferencích
Fakulta / Pracoviště MU

Lékařská fakulta

Citace
Popis Human embryonic stem cells are able to differentiate into every cell type in the human body making them a tremendous cell source for regenerative medicine. However, the production of human embryonic stem cell lines for clinical use is challenging as clean rooms, highly-qualified personnel, and standard operating procedures for both manufacture and quality control are required. Here we present the quality control of clinical-grade hESC lines that were derived in xeno-free, feeder-free conditions, and according to current good manufacturing practices. In-depth quality control provides essential information about the safety, pluripotency, and differentiation potential of lines and it is a necessary component of new clinical-grade hESC line establishment.
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