Informace o publikaci
Bacteriomes of dental plaques from teeth affected by apical periodontitis in patients with different periapical lesions: a pilot case-case study
| Autoři | |
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| Rok publikování | 2023 |
| Druh | Konferenční abstrakty |
| Fakulta / Pracoviště MU | |
| Citace | |
| Popis | Background: Apical periodontitis (AP), an acute or chronic inflammation of the root canal and periradicular tissues, is caused by oral bacteria. AP leads to the development of a periapical lesion such as periapical granuloma (G) and radicular cyst (RC). Unlike RCs, Gs tend to heal even without surgical root canal treatment. The role of oral bacteria in the process of G and RC development is unclear. Objective: To compare the bacteriomes of dental plaque from teeth affected by AP between patients with G and those with RC. Methods: Dental plaque swabs were collected from 51 patients with AP+G and 54 patients with AP+RC. All patients were clinically, radiographically, and histopathologically examined. Microbial DNA was isolated from swabs and negative controls (NC=42, DNA-free water) using the QIAamp DNA Mini Kit; isolates were spiked with a mock community. 16S rRNA sequencing was performed on the Illumina MiSeq instrument with ?5000 reads per sample. Results: In total, 104 dental plaques were found highly abundant for the content of bacterial DNA (one sample from a patient with AP+RC had a similar profile with NCs). The number of amplicon sequence variants and alpha diversities (Shannon index) were similar between cases (p>0.05), but significantly differed from NC (q<0.001, Kruskal-Wallis and Dunn´s test). Streptococcus, Actinomyces, Rothia, and Veillonella were the most common genera in dental plaques from teeth affected by AP. The relative abundance of the Gemella genus was significantly higher in samples from patients with AP+RC than in those with AP+G as well as NC (q=0.03, q<0.001, respectively, Kruskal-Wallis and Dunn´s test). Conclusion: Bacteriomes of dental plaques from patients with AP+G and AP+RC were similar in almost all observed characteristics, with Gemella being the only genus significantly more represented in patients with RC compared to those with G. |
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