R. sanguineus from Hungarian domestic dogs: identification and detection of tick-borne pathogens

• Autoři: GHODRATI Sajjad
• Rok publikování: 2023
• Druh: Konferenční abstrakty
• Citace: GHODRATI, Sajjad. R. sanguineus from Hungarian domestic dogs: identification and detection of tick-borne pathogens. 2023.
• Popis: R. sanguineus from Hungarian domestic dogs: identification and detection of tick-borne pathogens Sajjad Ghodrati 1,2 , Paulina Maria Lesiczka 3 , Luděk Žůrek 4,y,z, , Farkas Szekely xx , David Modrý 1,2,3 1 Department of Botany and Zoology, Faculty of Science, Masaryk University, Kotlářská 2, Brno, Czech Republic 2 Biology Centre, Institute of Parasitology, Czech Academy of Sciences, Branišovská 31, České Budějovice, Czech Republic 3 Department of Veterinary Sciences/CINeZ, Faculty of Agrobiology, Food and Natural Resources, Czech University of Life Sciences Prague, Kamýcka 129, Prague, Czech Republic Abstract Introduction: The brown dog tick, Rhipicephalus sanguineus sensu lato (s.l.) is a part of a complex of species with an unsettled species concept. A geographic pattern in the distribution of main lineages of ticks included in R. sanguineus s.l. was repeatedly discussed and major clades were commonly termed as “temperate lineage” (nowadays R. sanguineus s.str.) and a “tropical lineage” (nowadays R. linnaei). In Europe, R. sanguineus has been considered mainly a Mediterranean tick species with sporadic findings in northern countries (e.g. U.K., Ireland, Denmark, and Sweden). Due to global climatic changes, northward expansion of this thermophilic tick distribution range is observed, and it was reported as far north as Hungary. R. sanguineus is known as a vector of a range of pathogens of medical and veterinary importance. Including Anaplasma platys, Ehrlichia canis, B. vogeli, H. canis, R. conorii. A. platys is not yet reported in Hungary and there are only few reports of E. canis infection in the area. Objectives: The study reports the genetic identification of a series of R. sanguineus ticks collected from domestic dogs in central Hungary using nuclear and mitochondrial genetic markers and results of the detection of transmitted pathogens this recently established focus of R. sanguineus. Material and methods: Between 2020-2021 a total number of 1839 ticks collected by veterinarians were received from Hungary. All the ticks were morphologically identified using standard morphological keys. Of 1839 ticks 169 individuals were identified as R. sanguineus. A subset of 15 was chosen for photographing and molecular analysis for three markers (16S rDNA, 12S rDNA, Cox1). Results: Of 169 R. sanguineus ticks none were positive for A. platys and E. canis. However, sequencing of the amplicon for A. platys of the expected size from one dog revealed the presence of A. phagocytophilum (the agent of human granulocytic anaplasmosis).