Informace o publikaci
Cellular Distribution and Semi-Quantitative Changes of Delta Opioid Receptors in The Spinal Dorsal Horn of a Mouse Spared Nerve Injury Model
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| Rok publikování | 2024 |
| Druh | Konferenční abstrakty |
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| Popis | The primary sensory neurons (PSN) of the dorsal root ganglia (DRG) play a pivotal role in the afferentation of peripheral nociception into the spinal cord. The majority of the central branches of PSNs terminate on the neurons of the spinal dorsal horn (SDH). The aim of present experiments was to study semi-quantitative changes of DOR protein levels in lumbar (L-SDH) and cervical (C-SDH) of both sides (ipsilateral and contralateral) in a mouse model of neuropathic pain. We used unilateral mouse spared nerve injury model (n=8) with spared tibial nerve (SNIt). In sham-operated animals (n=8), the left sciatic nerve was only exposed. Both SNIt- and sham-operated animals were left to survive for 7 and 21 post-operation days (POD). Four mice without any operation were used as naive control. The spinal cord segments in the range of L3-L5, C4-C6 were dissected. A set of cryostat sections (12µm) was immunostained with polyclonal DOR primary antibody under the same conditions. Double immunostaining with IB4-FITC and DOR antibody was to distinguish DOR distribution in layers I and II. Levels of DOR-IF were similar in both C-SDH and L-SDH of naive mice. Sham or SNIt operations induced bilateral increases in DOR-IF in L-SDH at POD7 and POD21. The increased DOR-IF was found in C-SDH on ipsilateral side after sham, but on contralateral side after SNIt operation at POD7. However, no significant changes were found in C-SDH after sham or SNIt at POD21. The results indicate that both sham or SNIt operations resulted in bilateral changes in DOR protein levels in both C-SDH and L-SDH. |
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