Informace o publikaci
PHAGE TRAINING: ENHANCING BACTERIOPHAGE EFFICACY IN ANTIBIOTIC-INFUSED ENVIRONMENTS
| Autoři | |
|---|---|
| Rok publikování | 2024 |
| Druh | Konferenční abstrakty |
| Fakulta / Pracoviště MU | |
| Citace | |
| Popis | Viruses of microbes (bacteriophages) are an ubiquitous entity present in our environment. From the beginning of the previous century humankind looking for their utilization in medicine. The medicinal approach counts only with strictly lytic phages, which lyses the target pathogenic bacteria without adverse effects on the human host. So the second most important criterium is the phage ability to lyse the selected bacterial strain. Unfortunately the phage banks may not always contain the right phages and the isolation of the new lytic phages could be tedious and not fast enough for the patient. Another obstacle is that the lytic ability of phage could change during the therapeutic process, because of emergence of the phage resistance. Phage training could be a solution for these situations. It is a process of adaptive laboratory evolution, where the phages should acquire the ability to lyse the selected bacteria. Our training approach is based on Phage-Antibiotic Synergy effect (PAS). The phage-antibiotic interactions could lead to an antagonism or synergy, where is the bacteria more efficiently eradicated. Both of these situation were used for the training of therapeutic phages. Resulting phages were tested for their efficiacy against the parental strain of S. Aureus and the best clones were isolated and sequenced by Nanopore technology. Genome changes were evaluated in comparison to the parental phage. Also the evolved bacteria were isolated, tested for their identity and resistance to the parental phage. The identifiaction of these changes could reveal a new insights into the phage-antibiotic function and evolution. |
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