Informace o publikaci
Crystal structure of a cytokinic-glucoside-specific beta-glucosidase from Zea mays
Autoři | |
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Rok publikování | 2000 |
Druh | Článek v odborném periodiku |
Časopis / Zdroj | Biologické listy |
Fakulta / Pracoviště MU | |
Citace | |
Obor | Genetika a molekulární biologie |
Klíčová slova | crystal structure |
Popis | Zm-p60.1, a cytokinin-glucoside-specific b-glucosidase from maize, is a key enzyme involved in plant development and growth. It has been over-expressed in soluble form from Escherichia coli with a His tag at its N-terminus, the recombinant protein has been purified and crystallized at room temperature using PEG 4000 as main precipitant. A flash-annealed monoclinic crystal diffracted to high resolution (beyond 2 A) with space group P21, and unit-cell parameters a = 55.66 A, b = 110.72 A, c = 72.94 A, b = 92.10 deg. Resulting crystal structure was solved by molecular replacement. |
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