Informace o publikaci
IMMUNOHISTOCHEMICAL DIFFERENCES IN THE ECM CONTENT ALONGSIDE AFFERENT AND MOTOR AXONS IN RELATION TO THE POSITION OF THE DORSAL ROOT GANGLION
| Autoři | |
|---|---|
| Rok publikování | 2000 |
| Druh | Článek ve sborníku |
| Konference | 37th Proceedings of Czech Society of Histochemistry and Cytochemistry |
| Fakulta / Pracoviště MU | |
| Citace | |
| Obor | Neurologie, neurochirurgie, neurovědy |
| Popis | To reveal different microenvironmental conditions for motor and afferent axons we have analyzed immunohistochemically some ECM components and adhesion molecules (laminin-2, -3, fibronectin, thrombospondin, tenascin, chondroitin sulfate, and HNK-1) in the spinal roots and branches of the spinal nerve.Our results revealed no differences in the fibronectin immunofluorescence between dorsal and ventral roots as well as dorsal and ventral branches of the spinal nerve. There were also no differences between ventral and dorsal branches in the thrombospondin occurrence peripheral to DRG, and no differences were found between ventral and dorsal root contents of both laminin-2 and -3 central to the DRG. A higher immunofluorescence staining for HNK-1 and CS-56 was proved in the dorsal root as well as dorsal branch of the spinal nerve in contrast to the ventral root and branch. The greater amount of thrombospondin and tenascin molecules was disclosed in the dorsal roots than ventral ones, while higher immunofluorescence staining for laminin-2 and -3 was found in the dorsal in contrast to ventral branches of the peripheral nerves. On the other hand, tenascin molecules were more abundant in the ventral branches of the spinal nerve than the dorsal ones. The results of quantitative immunofluorescence analysis suggest some differences in the content of ECM along the afferent and motor axons that could be involved in the origin of different conditions for the axon regrowth after injury of the dorsal and ventral roots as well as the spinal nerves. |
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