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Electrochemical investigation of rabbit liver metallothionein at a mercury electrode

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TRNKOVÁ Libuše KIZEK René PALEČEK Emil

Rok publikování 2001
Druh Článek ve sborníku
Konference XVIth International Symposium on Bioelectrochemistry and Bioenergetics
Fakulta / Pracoviště MU

Přírodovědecká fakulta

Citace
Obor Elektrochemie
Klíčová slova rabbit liver metallothionein;constant current chronopotentiometric stripping analysis (CPSA); catalytic hydrogen evolution
Popis In this contribution we have studied a rabbit liver metallothionein by constant current chronopotentiometric stripping analysis (CPSA) with hanging mercury drop electrode. The highest sensitivity of the MT determination was obtained with CPSA. In (dE/dt)-1 - E coordinates rabbit liver metallothionein gives the well developed peak H. This H peak, produced due to catalytic hydrogen evolution at highly negative potentials, is sharp and reproducible [4]. The shape, height, and position of the H peak were studied under varying experimental conditions - pH, supporting electrolyte composition, time of accumulation, temperature, concentrations of MT and buffers, and the presence or absence of oxygen and metals in solutions.
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