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Silver Electrode as a Sensor for Determination of Zinc in Cell Cultivation Medium

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KIZEK René TRNKOVÁ Libuše ŠEVČÍKOVÁ Sabina ŠMARDA Jan JELEN František

Rok publikování 2002
Druh Článek v odborném periodiku
Časopis / Zdroj Analytical Biochemistry
Fakulta / Pracoviště MU

Přírodovědecká fakulta

Citace
Obor Elektrochemie
Klíčová slova silver electrode; cyclic voltammetry; zinc in growth medium; inducible gene expression; BM2 cells; immunoblotting
Popis Use of the silver electrode as a sensor for monitoring of zinc in cell growth medium is described. Zinc at siver electrodes provides specific voltammetric signal, which is affected by solution components. Signlas of zinc ions in phosphate buffer solutions with and without cell growth meidum were compared. Common DMEM cell culture medium was used for the cultivation of a cell line of v-myb-transformed chicken monoblasts and its variants expressing v-jun and c-jun in a zinc dependent manner. Electrochemical results showed zinc concentrations in the medium coincide very well with the jun expression. With respect to the low toxicity of xilver for eukaryotic cells, silver electrodes represent promising tools for the determination of zinc concentrations in vivo without the potential risk of a cell culture damage.
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