Informace o publikaci

Characterization of yellow-pigmented and motile enterococci isolated from intestines of the garden snail <I>Helix aspersa</I>

Logo poskytovatele
Autoři

ŠVEC Pavel DEVRIESE Luc SEDLÁČEK Ivo BAELE Margo VANCANNEYT Marc HAESEBROUCK Freddy SWINGS Jean DOŠKAŘ Jiří

Rok publikování 2002
Druh Článek v odborném periodiku
Časopis / Zdroj Journal of Applied Microbiology
Fakulta / Pracoviště MU

Přírodovědecká fakulta

Citace
www
Obor Mikrobiologie, virologie
Klíčová slova Bacterial Typing Techniques; DNA; Enterococcus; classification;Helix (Snails); Nucleic Acid Hybridization; Pigments/metabolism;16S Ribosomal RNA
Popis AIMS: Enterococci associated with garden snails (Helix aspersa) were studied in order to obtain reliable species identification and characterization. METHODS AND RESULTS: Twelve yellow-pigmented and motile enterococci, isolated from the intestines of garden snails, were phenotypically close to Enterococcus casseliflavus, but they showed certain unusual biochemical characteristics. tRNA intergenic length polymorphism analysis (tDNA-PCR) divided all strains studied into two groups, in full agreement with biochemical test results. 16S rDNA sequencing, DNA base composition analysis and DNA-DNA hybridization results showed unambiguously that the enterococci studied belonged to the species Ent. casseliflavus. The representative strains of described ecovars were deposited in the Czech Collection of Microorganisms (CCM) as Ent. casseliflavus CCM 4868, 4869, 4870 and 4871. CONCLUSIONS: Enterococcus casseliflavus associated with garden snails can be subdivided into groups. SIGNIFICANCE AND IMPACT OF THE STUDY: Enterococcus casseliflavus differs from other enterococcal species in that it is typically associated with plants, soil, water and invertebrate animals. The different groups that can be found in these widely occurring bacteria are possibly source-specific ecovars, as exemplified by the Ent. casseliflavus inhabiting the intestines of snails.
Související projekty:

Používáte starou verzi internetového prohlížeče. Doporučujeme aktualizovat Váš prohlížeč na nejnovější verzi.

Další info