Informace o publikaci
Investigating the genetic basic of Gyrodactylus salaris resistance in Atlantic Salmon (Salmo salar)
| Autoři | |
|---|---|
| Rok publikování | 2003 |
| Druh | Článek ve sborníku |
| Konference | Bulletin of the Scandinavian Society for Parasitology, Proceedings of the XXI. Symposium of the Scandinavian Society for Parasitology |
| Fakulta / Pracoviště MU | |
| Citace | |
| Obor | Genetika a molekulární biologie |
| Klíčová slova | monogenea; gyrodactylus salaris; FIP-2; VHSV |
| Popis | Gyrodactylus salaris Malmberg, 1957, an ectoparasite living on the skin and fins of salmonids, has had a catastrophic effect on wild Atlantic salmon (Salmo salar L.) stocks in Norway. Understanding the immune response, and the basis for observed resistance in some salmon populations, will play an important part in controlling this parasite. An overview of the experimental approach taken to address these aspects, and some preliminary results obtained, will be presented. Development of molecular markers linked to resistance could be used to introduce heritable resistance to parasites into commercial salmon populations through marker assisted selection methods. Two types of family groups were produced, from Scottish salmon susceptible to G. salaris and from resistant Russian River Neva salmon, and will be used to elucidate the molecular basis for variation in resistance to G. salaris, and to study mechanisms involved in host-parasite interaction. Fish gene expression is being analysed; genes that are stimulated or suppressed during infection, and, particularly genes that show differences in expression between resistant and susceptible fish, are being characterised. QTL markers linked to resistance will be identified. A number of differentially expressed products from G. salaris infected and control salmon have been isolated using differential display and subtractive hybridisation techniques, two of which are presented here. The first contains regions highly similar to the human, and chicken homolog, of 14.7K-interacting protein 2 (FIP-2), thought to play a role in tumour necrosis factor signaling. This gene was upregulated in G. salaris infected fish. The second product showed high similarities to a gene induced following VHSV infection of fish. The whole gene has not been fully characterised previously. This product was downregulated in G. salaris infected fish. |
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