Informace o publikaci
Application of Elimination Voltammetry to the Resolution of Adenine and Cytosine Signals in Oligonucleotides. I. Homo-oligodeoxynucleotides dA9 and dC9
| Autoři | |
|---|---|
| Rok publikování | 2003 |
| Druh | Článek v odborném periodiku |
| Časopis / Zdroj | Electroanalysis |
| Fakulta / Pracoviště MU | |
| Citace | |
| Obor | Elektrochemie |
| Klíčová slova | Reduction of adenine and cytosine in oligonucleotides; mercury electrode; elimination voltammetry with linear scan; dC9 and dA9 |
| Popis | Elimination voltammetry with linear scan (EVLS) has been applied to the resolution of reduction signals of adenine and cytosine in short synthetic homo-oligodeoxynucleotides (dA9 and dC9). In comparison with the common electrochemical methods (linear sweep, differential pulse, and square wave voltammetry) EVLS enables to resolve the overlapped signals by using the function which eliminates the charging and kinetic currents ( , ) and conserves the diffusion current ( ). For the adsorbed electroactive substance, this elimination function gives a well readable peak-counterpeak which has successfully been utilized to the analysis of overlapped reduction signals of adenine and cytosine on hanging mercury drop electrode (HMDE). The height and potential of signals studied were affected by the dC9/dA9 ratio, the time of accumulation, the stirring speed during the adsorption, and pH. Our results showed that EVLS in connection with the adsorption procedure is a useful tool for qualitative and quantitative studies of short oligonucleotides. |
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