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Informace o publikaci
Comparison of the bioluminescence of Photorhabdus species and subspecies type strains
Autoři | |
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Rok publikování | 2004 |
Druh | Článek v odborném periodiku |
Časopis / Zdroj | Folia Microbiologica |
Fakulta / Pracoviště MU | |
Citace | |
Popis | Natural bioluminescence of genus Photorhabdus, bacterial symbionts of entomopathogenic nematodes was studied. Bioluminescence of all recently accepted Photorhabdus species and subspecies type strains was measured using a commercial luminometer. Optimal conditions for the measurement and comparison of all species and subspecies are described. Cultures of all tested strains emitted reliably measurable bioluminescence, with characteristic level and kinetics for each strain. Bioluminescence of all strains was significantly higher at 37 C when compared with 25 C at the beginning of the measurement, no effect of bacterial concentration on the intensity of bioluminescence was observed in our experimental conditions. The technique described can provide reliable and quick information for determination of Photorhabdus taxons. |