Informace o publikaci
Development of the piezoelectric biosensor for acetochlor detection
| Autoři | |
|---|---|
| Rok publikování | 2003 |
| Druh | Článek v odborném periodiku |
| Časopis / Zdroj | Analytical Letters |
| Fakulta / Pracoviště MU | |
| Citace | |
| Obor | Biochemie |
| Klíčová slova | Piezoelectric sensor; Immunosensor; Acetochlor |
| Popis | The piezoelectric immunosensor for the determination of acetochlor was developed. The surface of gold electrodes of piezoelectric quartz crystals was modified by self-assembled thiolayers using either 4-aminothiophenol or dithiobis(succinimidyl propionate). In the next step, the modified surface was used for the coupling of acetochlor-protein conjugates. Acetochlor was conjugated to ovalbumin using either thiopropionic acid or acetylthiosuccinimidyl anhydride (AMSA). The acetochlor-modified crystals were used for characterization of the anti-acetochlor polyclonal antibody (Ab). The kinetic rate and equilibrium constants were compared for both types of immobilization. For acetochlor immobilized through AMSA, the dissociation rate constant was 20-times lower. The possibility of using this system for the competitive determination of free acetochlor in water was further studied. The detection limit (10% decrease of relative binding of the antibody) was 0.20 mg/l). The piezoelectric crystals were used repeatedly, 100 mM formic acid served for regeneration of the sensing surface. The total time for one measurement was about 30 min including 15 min pre-incubation of antibody with sample, 10 min binding reaction and 4 min regeneration. |
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