Informace o publikaci
Changes in the protein profiles and activities of antioxidant enzymes in fat body of Galleria mellonella in response to boric acid
| Autoři | |
|---|---|
| Rok publikování | 2006 |
| Druh | Článek ve sborníku |
| Konference | VIIIth European Congress of Entomology |
| Fakulta / Pracoviště MU | |
| Citace | |
| Popis | Alterations in antioxidative response and protein profiles associated with lipid peroxidation level were investigated in fat body of the greater waxmoth, Galleria mellonella (L.) after boric acid (156, 620, 1250 and 2500 ppm) treatment in final instars (VIIth) and pupae. Sodium dodecyl sulphate–polyacrylamide gradient gel electrophoresis (SDS-PAGGE) was used to determine the protein profiles in fat body. Approximately 16-20 protein fractions in the fat body of VIIth instar larvae and pupae were separated and some others were observed in a poor resolution. Protein concentrations in larval fat body was lesser than in pupae. Except apoLp I, similar fractions as in pupal fat body were also obtained in larvae. Storage proteins of molecular weight 72-84 kDa were significantly more abundant in the pupal fat body than in larval fat body. Malondialdehyde (MDA) content was significantly increased in the fat body of larvae and pupae at high concentrations of boric acid. Superoxide dismutase (SOD) activities were significantly increased while catalase (CAT) activities were significantly decreased in larval fat body at all concentrations of boric acid. However, boric acid also resulted in significant increased CAT activities in pupal fat body. Highest concentrations of boric acid resulted in significantly decreased GST activity in larval and pupal fat body. At lowest concentration, boric acid significantly increased the GPX activity in the larval and pupal fat body in comparison to control. There is a significant correlation between SOD activities and MDA content in the larval fat body of the insect. This study suggests that boric acid-induced oxidative stress may be causative factor in weakening of larval and pupal antioxidative defense system and may cause changes in fat body protein profiles. Key words: G. mellonella, boric acid, proteins, antioxidant enzymes, fat body, malondialdehyde |