Informace o publikaci
Minimization of adsorption in capillary zone electrophoresis of proteins and neuroprotective peptides
| Název česky | Minimalizace adsorpce proteinů a neuroprotektivních peptidů v kapilární zónové elektroforéze |
|---|---|
| Autoři | |
| Rok publikování | 2005 |
| Druh | Článek ve sborníku |
| Konference | Collection Symposium Series, Biologically Active Peptides |
| Fakulta / Pracoviště MU | |
| Citace | |
| Obor | Analytická chemie, separace |
| Klíčová slova | Capillary electrophoresis; Coating; Electroosmotic flow; Polyethyleneimine; 3glycidoxypropyltrimethoxysilane; 3aminopropyltriethoxysilane; Linear polyacrylamide |
| Popis | Four types of coating procedures suitable for protein and peptides separation: covalent coating using polyethyleneimine (PEI), 3-glycidoxy-propyltrimethoxysilane (GPTMS), 3-aminopropyltriethoxysilane (APS) and a new approach of dynamic coating with linear polyacrylamide (LPA) have been studied. The capillary surface was characterized by determination of the electroosmotic flow (EOF); for optimization a model mixture of proteins (cytochrome C, lysozyme and ribonuclease A) was used. Separation of a neuroprotective peptide [Gly-14]humanin was demonstrated in the optimized system. |