Informace o publikaci
Electroanalytical techniques for determination of flavonoids
| Autoři | |
|---|---|
| Rok publikování | 2007 |
| Druh | Článek ve sborníku |
| Konference | EuroMedlab Amsterdam 2007 |
| Fakulta / Pracoviště MU | |
| Citace | |
| Obor | Elektrochemie |
| Klíčová slova | flavonoids; voltammetry; cytochroms |
| Popis | Introduction. Flavonoids are a group of polyphenolic compounds widely distributed throughout the plant kingdom. They are characterized by the presence of two benzene rings that are linked by a 3-carbon bridge or by a pyrane or pyrone ring. Flavonoids have been found to exhibit a wide spectrum of pharmacological properties, including antioxidative, antiallergic, antiinflammatory, antidiabetic, antiviral, and antineoplastic activities. Their anti-cancer effect has been also investigated. Materials and methods. Electrochemical measurements were performed with AUTOLAB Analyser. Results. Due to high abundance of flavonoids in human diet, we decided not only to optimize the square wave voltammetry for the detection of quercetin, quercitrin, rutin, chrysin and diosmin but also to examine them as potential inducers of cytochrome(s). Based on the results obtained, the most suitable conditions for determination of the flavonoids were as follows: frequency 180 Hz, step potential 1.95 mV/s and phosphate buffer of pH 7 as supporting electrolyte. The detection limits of the flavonoids were about tens of nM. After that we examined the certain flavonoids as potential inducers of cytochrome(s) P450. The data obtained demonstrate the necessity of taking into account not only ability of flavonoids to bind to Ah receptor (induction factor) but also to concentrate on their distribution and metabolism in the body. Conclusions. Electrochemical techniques represent advantageous possibility of miniaturisation and simultaneous analysis in vivo without harming or killing of living organism in comparison with the commonly methods used for investigation of flavonoids. |