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The AtGenExpress global stress expression data set: protocols, evaluation and model data analysis of UV-B light, drought and cold stress responses
Autoři | |
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Rok publikování | 2007 |
Druh | Článek v odborném periodiku |
Časopis / Zdroj | The Plant Journal |
Fakulta / Pracoviště MU | |
Citace | |
www | http://www3.interscience.wiley.com/cgi-bin/fulltext/118488525/HTMLSTART |
Obor | Genetika a molekulární biologie |
Klíčová slova | AtGenExpress; bioinformatics; gene expression; microarray; abiotic stress |
Popis | The tolerance responses of plants to many abiotic stresses are conjectured to be controlled by complex gene networks. In the frame of the AtGenExpress project a comprehensive Arabidopsis thaliana genome transcript expression study was performed using the Affymetrix ATH1 microarray in order to understand these regulatory networks in detail. In contrast to earlier studies, we subjected, side-by-side and in a high-resolution kinetic series, Arabidopsis plants, of identical genotype grown under identical conditions, to different environmental stresses comprising heat, cold, drought, salt, high osmolarity, UV-B light and wounding. Here we describe the technical performance of the experiments. We also present a general overview of environmental abiotic stress-induced gene expression patterns and the results of a model bioinformatics analysis of gene expression in response to UV-B light, drought and cold stress. |
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