Informace o publikaci
Improved Electrochemical Detection of Purine Nucleobases at Mechanically Roughened Edge-Plane Pyrolytic Graphite Electrode
| Autoři | |
|---|---|
| Rok publikování | 2009 |
| Druh | Článek v odborném periodiku |
| Časopis / Zdroj | ELECTROANALYSIS |
| Fakulta / Pracoviště MU | |
| Citace | |
| Obor | Elektrochemie |
| Klíčová slova | Voltammetry; Adsorptive stripping; Anodic stripping; Carbon electrodes; Mechanically roughened electrodes; Edge-plane pyrolytic graphite; DNA; Nucleobases; Oligonucleotides; Copper; Inverted drop microcell |
| Popis | Mechanically grinded edge-plane pyrolytic graphite electrode (g-PGEe) was applied in voltammetric analysis of purine nucleobases, acid-hydrolyzed synthetic oligodeoxynucleotides and a nonhydrolyzed plasmid DNA. Properties of the mechanically grinded electrodes in these analytical applications were compared with some other carbon electrode types. We show that the electrode surface grinding with 15-mu M SiC particles resulted in a remarkable improvement of oxidation signals of purine bases with no addition of copper ions. Addition of the copper ions., causing a strong enhancement of the purine oxidation responses at fine-polished carbon electrodes, had only small effect on the purine signals at the g-PGEe. On the other hand, the g-PGEe appeared less suitable for the ex situ AdTS voltammetric measurements of nonhydrolyzed plasmid DNA, compared to freshly peeled basal plane pyrolytic graphite electrode. |