Informace o publikaci
Interpretation of surface structures in frozen-etched protoplasts of yeasts.
| Autoři | |
|---|---|
| Rok publikování | 1969 |
| Druh | Článek v odborném periodiku |
| Časopis / Zdroj | Exp Cell Res. |
| Fakulta / Pracoviště MU | |
| Citace | |
| www | http://apps.isiknowledge.com/CitedFullRecord.do?product=WOS&db_id=WOS&SID=S2l3Ool |
| Obor | Morfologické obory a cytologie |
| Klíčová slova | yeast protoplasts, freeze-fracture, transmission electron microscopy |
| Popis | The fine structure of protoplast surface of Saccharomyces cerevisiae was studied by means of the freeze-etching technique. The protoplasts frozen in an osmotic stabilizer are enveloped in a non-etchable layer. This layer is related to the artificial eucteticum formed during the freezing and vizualized after etching. In the replicas there were found prints in the non-etchable envelope which are complementary to the protoplast surface. The exposed protoplast surface reveals invaginations and is covered with globules of about 100 A in diameter. A part of them is arranged into a hexagonal pattern. Remnants of the original cell wall were not found and henceforth yeast protoplasts are to be considered as completely devoid of the cell wall |