Publication details
Interpretation of surface structures in frozen-etched protoplasts of yeasts.
| Authors | |
|---|---|
| Year of publication | 1969 |
| Type | Article in Periodical |
| Magazine / Source | Exp Cell Res. |
| MU Faculty or unit | |
| Citation | |
| Web | http://apps.isiknowledge.com/CitedFullRecord.do?product=WOS&db_id=WOS&SID=S2l3Ool |
| Field | Morphological specializations and cytology |
| Keywords | yeast protoplasts, freeze-fracture, transmission electron microscopy |
| Description | The fine structure of protoplast surface of Saccharomyces cerevisiae was studied by means of the freeze-etching technique. The protoplasts frozen in an osmotic stabilizer are enveloped in a non-etchable layer. This layer is related to the artificial eucteticum formed during the freezing and vizualized after etching. In the replicas there were found prints in the non-etchable envelope which are complementary to the protoplast surface. The exposed protoplast surface reveals invaginations and is covered with globules of about 100 A in diameter. A part of them is arranged into a hexagonal pattern. Remnants of the original cell wall were not found and henceforth yeast protoplasts are to be considered as completely devoid of the cell wall |