Informace o publikaci
Actin cortical cytoskeleton and cell wall synthesis in regenerating protoplasts of the Saccharomyces cerevisiae actin mutant DBY 1693
| Autoři | |
|---|---|
| Rok publikování | 1995 |
| Druh | Článek v odborném periodiku |
| Časopis / Zdroj | Microbiology 141 (1995), 1289-1299. Impact Factor =3.025 |
| Fakulta / Pracoviště MU | |
| Citace | |
| www | http://apps.isiknowledge.com/full_record.do?product=WOS&search_mode=AuthorFinder&qid=12&SID=Q1la3NM8N23kn3cADeO&page=26&doc=255 |
| Obor | Mikrobiologie, virologie |
| Klíčová slova | : SACCHAROMYCES CEREVISIAE; ACTIN MICROFILAMENTS; WALL SYNTHESIS; BETA-(1-]3)-D-GLUCAN MICROFIBRILS; ELECTRON MICROSCOPY |
| Popis | Relation between actin and wall synthesis was studied in protoplasts of actin mutant act1-1. Since protoplasting also disturbs actin cytoskeleton, these mutant protoplasts had a double error in their actin cytoskeleton. In the period between the onset of wall synthesis and completion of the wall, protoplasts at permissive temperature showed even distribution of actin patches over the surface on which a new cell wall synthesized. After wall completion, actin patches partially disappeared, but then re-appeared, accumulated in regions of polarized growth. This reminded the pattern of actin patches in mutant cells. Electron microscopy of freeze-etched replicas revealed finger-like invaginations of the plasma membrane in mutant cells and their protoplasts showing similar distribution to actin patches detected by fluorescence microscopy. A hypothesis explaining the role of actin patches/finger-like invaginations of plasma membrane in the synthesis of beta-1,3-D-glucan wall microfibrils is presented. |