Pentapeptide-modified poly(N,N-diethylacrylamide) hydrogel scaffolds for tissue engineering

• Autoři: HORÁK Daniel; MATULKA Kamil; HLÍDKOVÁ Helena; LAPČÍKOVÁ Monika; BENEŠ Milan J.; JAROŠ Josef; HAMPL Aleš; DVOŘÁK Petr
• Rok publikování: 2011
• Druh: Článek v odborném periodiku
• Časopis / Zdroj: Journal of Biomedical Materials Reseach Part B: Applied Biomaterials
• Fakulta / Pracoviště MU: Lékařská fakulta
• Doi: http://dx.doi.org/10.1002/jbm.b.31832
• Obor: Genetika a molekulární biologie
• Klíčová slova: poly(NN-diethylacrylamide); porosity; YIGSR; peptide; embryonic stem cells; hydrogel
• Popis: Poly(N,N-diethylacrylamide) (PDEAAm) hydrogel scaffolds were prepared by radical copolymerization of N,Ndiethylacrylamide (DEAAm), N,N0-methylenebisacrylamide and methacrylic acid in the presence of (NH4)2SO4 or NaCl. The hydrogels were characterized by low-vacuum scanning electron microscopy in the water-swollen state, water and cyclohexane regain, and by mercury porosimetry. The pentapeptide, YIGSR-NH2, was immobilized on the hydrogel. Human embryonic stem cells (hESCs) were cultured with the hydrogels to test their biocompatibility. The results suggest that the PDEAAm hydrogel scaffolds are nontoxic and support hESC attachment and proliferation, and that interconnected pores of the scaffolds are important for hESC cultivation. Immobilization of YIGSR-NH2 pentapeptide on the PDEAAm surface improved both adhesion and growth of hESCs compared with the unmodified hydrogel. The YIGSRNH2- modified PDEAAm hydrogels may be a useful tool for tissue-engineering purposes.

Související projekty

• Centrum chemické genetiky
• Funkční a molekulární charakteristiky nádorových a normálních kmenových buněk - identifikace cílů pro nová terapeutika a terapeutické strategie