Heterogeneity in the kinetics of nuclear proteins and trajectories of substructures associated with heterochromatin

• Autoři: STIXOVÁ Lenka; BÁRTOVÁ Eva; MATULA Pavel; DANĚK Ondřej; LEGARTOVÁ Soňa; KOZUBEK Stanislav
• Rok publikování: 2011
• Druh: Článek v odborném periodiku
• Časopis / Zdroj: Epigenetics & Chromatin
• Fakulta / Pracoviště MU: Fakulta informatiky
• www: http://www.epigeneticsandchromatin.com/content/4/1/5
• Doi: http://dx.doi.org/10.1186/1756-8935-4-5
• Obor: Genetika a molekulární biologie
• Klíčová slova: protein dynamics HP1b BMI1 TRF1 trajectory heterochromatin
• Popis: We show that protein dynamics are influenced by many factors and events, including nuclear pattern and transcription activity. A slower recovery after photobleaching was found when proteins, such as HP1b, BMI1, TRF1, and others accumulated at specific foci. In identical cells, proteins that were evenly dispersed throughout the nucleoplasm recovered more rapidly. Distinct trajectories for HP1b, BMI1, and TRF1 were observed after hyperacetylation or suppression of transcription. The relationship between protein trajectory and transcription level was confirmed for telomeric protein TRF1, but not for HP1b or BMI1 proteins. Moreover, heterogeneity of foci movement was especially observed when we made distinctions between centrally and peripherally positioned foci. Based on our results, we propose that protein kinetics are likely influenced by several factors, including chromatin condensation, differentiation, local protein density, protein binding efficiency, and nuclear pattern. These factors and events likely cooperate to dictate the mobility of particular proteins.

Související projekty

• Dynamika a organizace chromosomů během buněčného cyklu v normě a patologii