Trajectories and nuclear arrangement of PML bodies are influenced by A-type lamin deficiency

• Autoři: STIXOVÁ Lenka; MATULA Pavel; KOZUBEK Stanislav; CMARKO Dušan; GOMBITOVÁ Adriana; RAŠKA Ivan; BÁRTOVÁ Eva
• Rok publikování: 2012
• Druh: Článek v odborném periodiku
• Časopis / Zdroj: Biology of the Cell
• Fakulta / Pracoviště MU: Fakulta informatiky
• www: http://onlinelibrary.wiley.com/doi/10.1111/boc.201100053/abstract
• Doi: http://dx.doi.org/10.1111/boc.201100053
• Obor: Genetika a molekulární biologie
• Klíčová slova: ACUTE PROMYELOCYTIC LEUKEMIA; DOUBLE-STRAND BREAKS; HUMAN-CELLS; PROTEIN STRUCTURES; STEM-CELLS; RAR-ALPHA; RNA; TELOMERE; HETEROCHROMATIN; TRANSCRIPTION
• Popis: Background information Promyelocytic leukaemia (PML) bodies are specific nuclear structures with functional significance for acute promyelocytic leukaemia. In this study, we analysed the trajectories of PML bodies using single-particle tracking. Results We observed that the recovery of PML protein after photobleaching was ATP dependent in both wild-type (wt) and A-type lamin-deficient cells. The movement of PML bodies was faster and the nuclear area occupied by particular PML bodies was larger in A-type lamin-deficient fibroblasts compared with their wt counterparts. Moreover, dysfunction of the LMNA gene increased the frequency of mutual interactions between individual PML bodies and influenced the morphology of these domains at the ultrastructural level. As a consequence of A-type lamin deficiency, PML protein accumulated in nuclear blebs and frequently appeared at the nuclear periphery. Conclusions We suggest that the physiological function of lamin A proteins is important for events that occur in the compartment of PML bodies. This observation was confirmed in other experimental models characterised by lamin changes, including apoptosis or the differentiation of mouse embryonic stem cells.

Související projekty

• Dynamika a organizace chromosomů během buněčného cyklu a při diferenciaci v normě a patologii