Informace o publikaci
Impedimetric Aptasensor for Thrombin Recognition Based on CD Support
| Autoři | |
|---|---|
| Rok publikování | 2012 |
| Druh | Článek v odborném periodiku |
| Časopis / Zdroj | Electroanalysis |
| Fakulta / Pracoviště MU | |
| Citace | |
| Doi | http://dx.doi.org/10.1002/elan.201200041 |
| Obor | Fyzikální chemie a teoretická chemie |
| Klíčová slova | DNA aptamers; Biosensors; Human thrombin; Electrochemical impedance spectroscopy; Gold CD-trodes |
| Popis | We report an aptamer biosensing array for thrombin detection by measuring the electrochemical impedance upon aptamer-protein formation at the surface of CD-trodes (GCDTs) in the presence of the redox couple [Fe(CN)6]3-/4-. GCDTs are fabricated from recordable compact discs that contain a fine gold layer. The biosensor is constructed by self-assembling of a thiol-modified thrombin binding aptamer (TBA) onto a GCDT surface. The sensor reveals good ligand specificity, recognition in a wide range of thrombin concentrations from 20 nM to 1 mu M with a limit of detection of 5 nM. |