Informace o publikaci

Sequential modifications in class II transactivator isoform 1 induced by lipopolysaccharide stimulate major histocompatibility complex class II transcription in macrophages

Autoři

DROZINA Gorazd KOHOUTEK Jiří NISHIYA Tadashi PETERLIN B Matija

Rok publikování 2006
Druh Článek v odborném periodiku
Časopis / Zdroj Journal of Biological Chemistry
Fakulta / Pracoviště MU

Středoevropský technologický institut

Citace
www http://www.jbc.org/content/early/2006/11/09/jbc.M608538200.full.pdf+html
Doi http://dx.doi.org/10.1074/jbc.M608538200
Obor Genetika a molekulární biologie
Klíčová slova ELONGATION-FACTOR-B; UBIQUITIN-MEDIATED PROTEOLYSIS; HUMAN-IMMUNODEFICIENCY-VIRUS; CREB BINDING-PROTEIN; TOLL-LIKE RECEPTORS; ACTIVATE TRANSCRIPTION; DIFFERENTIAL USAGE; DENDRITIC CELLS; CIITA FUNCTION; IFN-GAMMA
Popis By presenting antigenic peptides on major histocompatibility complex class (MHC) II determinants to CD4(+) T cells, macrophages help to direct the establishment of adaptive immunity. We found that in these cells, lipopolysaccharide stimulates the expression of MHC II genes via the activation of Erk1/2, which is mediated by Toll-like receptor 4. Erk1/2 then phosphorylates the serine at position 357, which is located in a degron of CIITA isoform 1 that leads to its monoubiquitylation. Thus modified, CIITA isoform 1 binds P-TEFb, which mediates the elongation of RNA polymerase II and co-transcriptional processing of nascent transcripts. This induction leads to the expression of MHC II genes. Subsequent polyubiquitylation results in the degradation of CIITA isoform 1. Thus, the signaling cascade from Toll-like receptor 4 to CIITA isoform 1 represents one connection between innate and adaptive immunity in macrophages.

Používáte starou verzi internetového prohlížeče. Doporučujeme aktualizovat Váš prohlížeč na nejnovější verzi.

Další info