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Project information
Application of adsorptive transfer and elemination techniques in oligonucleotides and nucleic acids analysis.

Project Identification
IAA1163201
Project Period
1/2002 - 12/2004
Investor / Pogramme / Project type
Academy of Sciences of the Czech Republic
  • Grants of distinctly investigative character focused on the sphere of research pursued at present particularly in the ASCR
MU Faculty or unit
Faculty of Science
Keywords
Oligonucleotides; nucleic acids; adsorptive transfer technique; elimination voltammetry nith linear
Cooperating Organization
Institute of Biophysics
  • Responsible person RNDr. František Jelen, CSc.

The principal aims of the joint research are (a) to develop new ways of detecting of nucleic acids bases using transfer technique in connection with elimination and stripping voltammetry, (b) to study short oligonucleotides in dependence on their sequence and length and (c) to use elimination voltammetry with linear scan and electrochemical impedance spectroscopy in oligonucleotides and nucleic acids analysis. Analysis will be performed at mercury and solid electrodes. It is expected that the results obtained in this project will be utilized as a simple sensitive and selective analytical procedures for the determination of nucleic acids and their components. The advatage of these analytical procedures consists in the possibility to modify electrodes by nucleic acids and oligonucleotides. Obtained results will be applied in various fields of biophysics, biochemistry and molecular biology.

Publications

Total number of publications: 54

2004

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