Electrochemical determination of lead and glutathione in a plant cell culture

• Authors: VACEK Jan; PETŘEK Jiří; KIZEK René; HAVEL Ladislav; KLEJDUS Bořivoj; TRNKOVÁ Libuše; JELEN František
• Year of publication: 2004
• Type: Article in Periodical
• Magazine / Source: Bioelectrochemistry
• MU Faculty or unit: Faculty of Science
• Field: Electrochemistry
• Keywords: Lead; Pb(II)-EDTA; glutathione; metallothionein; Voltammetry; Mercury electrode; Brdicka reaction
• Description: In this paper we established DPASV as the tool for the analysis of lead in plant cell culture. For the calibration procedure, lead in (Pb-EDTA) chelate has been used. The detection limit of lead was at 500 pM in phosphate buffer (pH 5.5), and 100 nM in prepared cells intracellular extract. For the determination of cystein-rich peptides, voltammetry in differential mode in cobalt(III)-containing ammonia buffer (Brdicka reaction) was used.

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