Project information
Epigenetic control of gene expression in malignant diseases
- Project Identification
- NC6987
- Project Period
- 1/2002 - 12/2004
- Investor / Pogramme / Project type
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Ministry of Health of the CR
- R&D of Oncology
- MU Faculty or unit
- Faculty of Informatics
- Keywords
- DNA-microarrays;cancer;chromosome structure;epigenetic regulation;cell differentiation
- Cooperating Organization
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Institute of Biophysics
- Responsible person doc. RNDr. Stanislav Kozubek, DrSc.
- Responsible person MUDr. Zdeněk Kořístek, Ph.D.
- Responsible person prof. MUDr. Jan Žaloudík, CSc.
Using DNA-microarray technology, the transcirptome maps will be determined for several normal and tumor cell populations in different stages of progression. Comparison ot maps should allow us to find out combinations of genes related to cancerogenesis. The structure of chromosomes will be inbestigated in interphase 3D-fixed nuclei by means of FISH and high-resolution cytometry. Changes of the structure re latively to the normal tissue xill be compared with changes of gene expression. Conclusion will be made on the relationship between the structure of chromosomes and expression of genes during cancerogenesis. It may give us new and relatively cheap method for the detection of oncogenic transformation in the specific tissue. The project will bring DNA-microarrays to the second laboratory in the Czech Republic, which will be important for the regional development of progressive tehnologies.
Publications
Total number of publications: 18
2005
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Methylation of histones in myeloid leukemias as a potential marker of granulocyte abnormalities
Journal of Leukocyte Biology, year: 2005, volume: 77, edition: 1
2004
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Automated image analysis in fluorescence microscopy: From isolated cells to tissues and microarray images
Biophysics of the Genome, year: 2004
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Colocalization of PML bodies and PML/RARalpha microspeckles with up- and down-regulated loci and changes of chromatin structure in APL leukemia cells
Biophysics of the Genome, year: 2004
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Cytological study of DNA methylation and histone methylation patterns in human cell lines
Biophysics of the Genome, year: 2004
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Different nuclear localization of aberrant and normal chromosome 8 homologues in intact or NaBt differentiated HT-29 cells
Biophysics of the Genome, year: 2004
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From Jenalumar to Ultrafast Microscopy of Living Cells
Biophysics of the Genome, year: 2004
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Gene expression in colorectal carcinoma determined by cDNA microarrays using a combination of several evaluation approaches.
Biophysics of the Genome, year: 2004
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Methylation of histone H3K9 in human blood cells and in granulocytes of patients with myeloid leukemia
Biophysics of the Genome, year: 2004
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Nové možnosti v diagnostice kolorektálního karcinomu s využitím technologie cDNA mikročipů
Klinická onkologie, year: 2004, volume: 6/17, edition: 6
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Relationship of HP1 proteins to centromeric heterochromatin characterized by the presence of H3(K9) and absence of H3(K4) dimethylation
Biophysics of the Genome, year: 2004